- [Resource Hub](/) - [Resource](/?resource-type=product-documentation#library) - CyteFinder II # CyteFinder II Imaging System CyteFinder II couples whole-slide fluorescence and brightfield imaging with image-guided single-cell retrieval, moving the rare cells it finds in liquid biopsy or tissue samples into high-depth sequencing. CyteFinder II runs as one connected workflow. It images a whole slide across seven fluorescence channels and brightfield. Then the integrated CytePicker Retrieval Module lifts individual cells off that slide for high-depth sequencing. RareCyte calls that workflow Pick-Seq. Every cell sent for sequencing traces back to a high-resolution image of where it sat, so multiomic data keeps its spatial context. Three configurations handle cells, tissue, or both. Together they cover CTC analysis, H&E and IHC imaging, high-resolution multiplexed tissue imaging, and differential expression on retrieved cells. Read or download to see: - Image-guided single-cell retrieval. The integrated CytePicker Retrieval Module picks individual cells at single-cell resolution through a 25 or 40 µm needle bore, then sends them to high-depth sequencing. - Seven fluorescence channels plus brightfield. Excitation ranges from 395 to 745 nm and emission from 438 to 800 nm, covering DAPI and BV421 through Qdot 800. Imaging runs on an sCMOS camera at 2560 × 2160 pixels and 6.5 µm pixel size, with 1.2 electrons of read noise and a five-color solid-state LED illuminator. - Whole-slide scanning built for discovery. A typical 20× whole-slide scan finishes in 6.5 minutes over a 1.5 cm × 1.5 cm area at five channels and 20 ms exposures, with image-based autofocus and a five-position automated turret. The stage holds two slides, loaded manually, with ±5.0 µm load repeatability. - Three configurations for liquid biopsy and tissue. Cell, Tissue, and Cell + Tissue builds carry objectives from 4× to 40×, with an optional 40× 0.95 NA, and support IF, IHC, and H&E on standard microscope slides or CytePicker chamber slides. [Download spec sheet](https://cdn.sanity.io/files/s3p6uqps/production/f426034dbc5fa8719efe9fa458e89675dc6cfa59.pdf) Your browser doesn't support embedded PDFs. [Download the CyteFinder II spec sheet](https://cdn.sanity.io/files/s3p6uqps/production/f426034dbc5fa8719efe9fa458e89675dc6cfa59.pdf) to view it. [Back to all resources](/#library) ## Related resources [Article](https://www.selectscience.net/product-news/measuring-versus-reading-transforming-the-field-of-quantitative-pathology?artid=58733) CyteFinder SelectScience ### Measuring versus reading – transforming the field of quantitative pathology [Read Article](https://www.selectscience.net/product-news/measuring-versus-reading-transforming-the-field-of-quantitative-pathology?artid=58733) [Spec Sheet](https://go.pardot.com/l/455452/2019-11-18/7mzyyz/455452/220141/TS20_103.pdf) CyteFinder ### CyteFinder II HT [View Spec Sheet](https://go.pardot.com/l/455452/2019-11-18/7mzyyz/455452/220141/TS20_103.pdf) [Publication](https://doi.org/10.1200/JCO.2026.44.16_suppl.5041) CyteFinder Journal of Clinical Oncology 2026 ### Clinical validation of circulating tumor cell synaptophysin and androgen receptor expression in metastatic prostate cancer phenotypes. [View Publication](https://doi.org/10.1200/JCO.2026.44.16_suppl.5041) [Video](https://www.youtube.com/watch?v=_oGC1KRVN2U) CyteFinder 2026 ### Dr. Pashtoon Kasi on the impact of circulating tumor cells on clinical research [Watch Video](https://www.youtube.com/watch?v=_oGC1KRVN2U) [Publication](https://doi.org/10.1038/s41587-026-03099-z) CyteFinder Nature Biotechnology 2026 ### mRNA vaccine immunity is enhanced by hepatocyte detargeting and not dependent on dendritic cell expression [View Publication](https://doi.org/10.1038/s41587-026-03099-z) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7618831/) CyteFinder Cancer Immunology Research 2026 ### Ex Vivo Immuno-Oncology Platform Reveals Spatial T Cell Infiltration Patterns Linked to ATR Inhibition Responses in High-Grade Serous Ovarian Cancer [View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC7618831/) [Publication](https://doi.org/10.1097/pai.0000000000001311) CyteFinder Applied Immunohistochemistry & Molecular Morphology 2026 ### Quantitative Assays for TROP2 Measurement in Breast Cancer and Comparison to H-Score [View Publication](https://doi.org/10.1097/pai.0000000000001311) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12881594/) CyteFinder Nature Communications 2026 ### Extracellular matrix mediates circulating tumor cell clustering in triple-negative breast cancer metastasis [View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12881594/) [Publication](https://doi.org/10.1016/j.ejso.2025.111357) CyteFinder European Journal of Surgical Oncology 2026 ### Circulating tumor cells predict relapse in head and neck mucosal squamous cell carcinoma [View Publication](https://doi.org/10.1016/j.ejso.2025.111357) [Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12805936/) CyteFinder Cancer Research Communications 2026 ### Surface Marker Identification to Capture Live Circulating Tumor Cells in Metastatic Triple-Negative Breast Cancer [View Publication](https://pmc.ncbi.nlm.nih.gov/articles/PMC12805936/) [Publication](https://doi.org/10.1158/1078-0432.CCR-25-0244) CyteFinder Clinical Cancer Research 2025 ### Pre-clinical Modeling of Navtemadlin Pharmacokinetics, Pharmacodynamics, and Efficacy in IDH-wildtype Glioblastoma [View Publication](https://doi.org/10.1158/1078-0432.CCR-25-0244) [Publication](https://doi.org/10.3390/cancers17162717) CyteFinder Cancers 2025 ### Comprehensive Longitudinal Linear Mixed Modelling of CTCs Illuminates the Role of Trop2, EpCAM, and CD45 in CTC Clustering and Metastasis [View Publication](https://doi.org/10.3390/cancers17162717)