- [Resource Hub](/)
- [Resource](/?resource-type=poster#library)
- Phenoplex spatial analysis of whole-slide colon adenocarcinoma imaged with Orion 13-plex one-round staining and imaging

# Phenoplex spatial analysis of whole-slide colon adenocarcinoma imaged with Orion 13-plex one-round staining and imaging

High-plex imaging of a whole tissue section usually trades marker count against the number of staining rounds, so this poster stains and images a 21.6 by 18.5 mm colon adenocarcinoma in a single 13-plex round on RareCyte’s Orion platform, then phenotypes its cells at single-cell resolution in Visiopharm’s Phenoplex.

Presented by Visiopharm and RareCyte.

Read or download to see:

- A whole-slide colon adenocarcinoma stained and imaged in a single round. A standard pathology slide was stained once with a 13-plex immune-oncology panel, then a 21.6 by 18.5 mm section was imaged in one round on RareCyte’s Orion spatial biology platform — capturing the whole tissue at single-cell resolution without the repeated staining steps that iterative high-plex methods require.

- Single cells read straight from the tissue, then mapped to compartments. Visiopharm’s Phenoplex segmented the whole-slide image into epithelial, stromal, and necrotic regions, called individual cells from DAPI-stained nuclei, and phenotyped tumor, T-cell, B-cell, and macrophage populations across the 13-plex panel — finding many T cells in the stroma but few infiltrating the epithelium.

- Spatial neighborhoods that separate PD-L1-positive from PD-L1-negative macrophages. Within the stromal region, nearest-neighbor and neighbor-count analyses out to a 30 µm radius showed that PD-L1+ macrophages sit in a different immune-cell neighborhood than PD-L1- macrophages, clustering with T cells and other macrophages while sitting farther from proliferative and regulatory T cells.

[Download poster](https://cdn.sanity.io/files/s3p6uqps/production/45652abe778b0db13bb49cd20b84dd4b4f851ba4.pdf)

Your browser doesn’t support embedded PDFs.
[Download the poster](https://cdn.sanity.io/files/s3p6uqps/production/45652abe778b0db13bb49cd20b84dd4b4f851ba4.pdf) to view it.

[Back to all resources](/#library)
## Related resources
[Article](https://www.selectscience.net/article/advanced-spatial-biology-sheds-new-light-on-therapeutic-research) Orion SelectScience
### Advanced spatial biology sheds new light on therapeutic research
[Read Article](https://www.selectscience.net/article/advanced-spatial-biology-sheds-new-light-on-therapeutic-research) [Article](https://www.selectscience.net/editorial-articles/bringing-spatial-biology-to-the-clinic-a-new-lens-on-cancer-biology/?&artID=58147) Orion SelectScience
### Bringing spatial biology to the clinic: “A new lens on cancer biology”
[Read Article](https://www.selectscience.net/editorial-articles/bringing-spatial-biology-to-the-clinic-a-new-lens-on-cancer-biology/?&artID=58147) [Article](https://www.selectscience.net/article/how-to-unlock-the-complexity-of-tumor-microenvironments) Orion SelectScience
### How to unlock the complexity of tumor microenvironments
[Read Article](https://www.selectscience.net/article/how-to-unlock-the-complexity-of-tumor-microenvironments) [Brochure](https://go.pardot.com/l/455452/2020-07-15/8p7453/455452/1606951069teslfZQe/BR21_101_Orion_Brochure_201202.pdf) Orion
### Orion Spatial Proteomics Platform
[View Brochure](https://go.pardot.com/l/455452/2020-07-15/8p7453/455452/1606951069teslfZQe/BR21_101_Orion_Brochure_201202.pdf) [Spec Sheet](https://pardot.rarecyte.com/l/455452/2026-02-24/965v3w/455452/1771949067AeuR6syb/Orion_HT_spec_sheet.pdf) Orion
### Orion HT
[View Spec Sheet](https://pardot.rarecyte.com/l/455452/2026-02-24/965v3w/455452/1771949067AeuR6syb/Orion_HT_spec_sheet.pdf) [Spec Sheet](https://pardot.rarecyte.com/l/455452/2025-10-31/961nyb/455452/1761933957WEJWJyeN/2025.07.14___SS___Orion__LE_.pdf) Orion
### Orion LE
[View Spec Sheet](https://pardot.rarecyte.com/l/455452/2025-10-31/961nyb/455452/1761933957WEJWJyeN/2025.07.14___SS___Orion__LE_.pdf) [Spec Sheet](https://go.pardot.com/l/455452/2020-07-15/8p742r/455452/1607104572nfrlJouR/DF_24_101_201203_Orion_Datafile.pdf) Orion
### Orion
[View Spec Sheet](https://go.pardot.com/l/455452/2020-07-15/8p742r/455452/1607104572nfrlJouR/DF_24_101_201203_Orion_Datafile.pdf) [Research Spotlight](/resources/spatial-profiling-immune-correlates-mss-crc/) Orion 2026
### Spatial Profiling Uncovers Immune Correlates of Durable Response in Phase 1/2 MSS Colorectal Cancer Trial
[Read Spotlight](/resources/spatial-profiling-immune-correlates-mss-crc/) [Publication](/resources/copanlisib-nivolumab-microsatellite-stable-colorectal/) Orion Nature Communications 2026
### Copanlisib in combination with nivolumab for microsatellite stable colorectal cancer: a phase 1/2 trial
[Read More](/resources/copanlisib-nivolumab-microsatellite-stable-colorectal/) [Publication](https://doi.org/10.1158/2159-8290.CD-26-0171) Orion Cancer Discovery 2026
### Spatial Integration of Protein and Chromosomal States Reveals Early Copy-Number Changes and Genotype-Associated Immune Neighborhoods in Serous Ovarian Cancer Evolution
[View Publication](https://doi.org/10.1158/2159-8290.CD-26-0171) [Publication](https://www.nature.com/articles/s41467-026-74933-w) Orion Nature Communications 2026
### Multiple human transgenes prolong survival of triple-carbohydrate knockout porcine kidney xenografts in nonhuman primates
[View Publication](https://www.nature.com/articles/s41467-026-74933-w) [Video](https://www.youtube.com/watch?v=KOiToZIGN0g) Orion 2026
### Dr. Tanjina Kader on mapping spatial proteomics and chromosomal changes on the same tissue section
[Watch Video](https://www.youtube.com/watch?v=KOiToZIGN0g)