Orion™: 17-plex single-step stain and imaging of colorectal adenocarcinoma
This SITC 2023 poster shows a whole FFPE section of invasive colorectal adenocarcinoma stained with a 17-plex immuno-oncology panel and imaged on the Orion system in a single staining and scanning process, mapping the tumor epithelium, its invasive border, and the surrounding immune microenvironment on one slide.
Presented by RareCyte.
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- A whole FFPE section of invasive colorectal adenocarcinoma, stained in one round and imaged in one scan at 17-plex. The section was stained with a 17-plex immuno-oncology biomarker panel and imaged on the Orion system in a single staining and scanning process, with tissue autofluorescence imaged and isolated as an additional channel that resolved the nerve fibers and vessels of a neurovascular bundle in the colon wall.
- Epithelial markers resolved the tumor from its glands to its invasive front. Cytokeratins (Pan-CK) and E-Cadherin marked adenocarcinoma cells invading beneath the mucosa, and at the infiltrating border — in contrast to other tumor regions — the Ki-67 proliferative fraction was much lower, E-Cadherin staining was reduced, and cytokeratins grew more pronounced in small clusters of malignant budding cells breaking off from the glands.
- The same panel phenotyped immune cells and read their checkpoints on individual cells. A dense collection of activated immune cells (CD45RO) between the cancer glands was sub-typed as mostly CD4+ T helper cells with FOXP3+ regulatory T cells scattered within, while interstitial macrophages co-expressing CD68 and CD163 were an important source of PD-L1, which binds PD-1 on T cells to suppress the anti-tumor immune response.
