Orion™: 17-plex single-step stain and imaging of normal lung section adjacent to non-small cell lung carcinoma
This AACR 2024 poster resolves normal lung microanatomy and its immune infiltrate from a 17-marker whole-slide scan of a section adjacent to a non-small cell lung carcinoma, with every marker stained in a single staining procedure and imaged in a single scan on the Orion instrument.
Presented by Navigate BioPharma.
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- A 17-marker panel, stained in one round and imaged in one scan. The FFPE lung section was stained with a 17-plex immunofluorescence (IF) panel in one staining round, followed by whole slide imaging with the Orion instrument in one imaging round; tissue autofluorescence was imaged and isolated as an additional fluorescence channel.
- Marker localization resolves epithelial polarity and subcellular compartments. In the bronchiolar epithelium E-Cadherin is prominently expressed in the basal part, while cytokeratin 7/8 detected by CK-CAM5.2 is more prominent in the apical part (figure 3); in the alveolus CD68 stains the cytoplasm of the alveolar macrophages, while CD163 marks the cell membrane (figure 2).
- The immune infiltrate is typed down to T cell subsets and proliferation state. The alveolar interstitial infiltrate is predominantly T cells (CD3d), with interspersed B cells (CD20) and macrophages (CD163) (figure 4); the T cells are a mixture of helper (CD4) and cytotoxic (CD8a) cells, scattered cells express nuclear FOXP3, a marker of T regulatory cells, and most FOXP3-positive cells are CD4 T cells (figure 6); proliferating cells express the cell cycle markers PCNA and/or Ki67, and the activation/memory marker CD45RO is present in many of them (figure 5).
