Phenoplex spatial analysis of whole-slide colon adenocarcinoma imaged with Orion 13-plex one-round staining and imaging

High-plex imaging of a whole tissue section usually trades marker count against the number of staining rounds, so this poster stains and images a 21.6 by 18.5 mm colon adenocarcinoma in a single 13-plex round on RareCyte’s Orion platform, then phenotypes its cells at single-cell resolution in Visiopharm’s Phenoplex.

Presented by Visiopharm and RareCyte.

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• A whole-slide colon adenocarcinoma stained and imaged in a single round. A standard pathology slide was stained once with a 13-plex immune-oncology panel, then a 21.6 by 18.5 mm section was imaged in one round on RareCyte’s Orion spatial biology platform — capturing the whole tissue at single-cell resolution without the repeated staining steps that iterative high-plex methods require.
• Single cells read straight from the tissue, then mapped to compartments. Visiopharm’s Phenoplex segmented the whole-slide image into epithelial, stromal, and necrotic regions, called individual cells from DAPI-stained nuclei, and phenotyped tumor, T-cell, B-cell, and macrophage populations across the 13-plex panel — finding many T cells in the stroma but few infiltrating the epithelium.
• Spatial neighborhoods that separate PD-L1-positive from PD-L1-negative macrophages. Within the stromal region, nearest-neighbor and neighbor-count analyses out to a 30 µm radius showed that PD-L1+ macrophages sit in a different immune-cell neighborhood than PD-L1- macrophages, clustering with T cells and other macrophages while sitting farther from proliferative and regulatory T cells.