Phenoplex™ spatial analysis of whole-slide colon adenocarcinoma imaged with Orion 13-plex one-round staining and imaging
High-plex imaging of a whole tissue section usually means staining it over several rounds, so this poster instead stains a 21.6 by 18.5 mm colon adenocarcinoma once with a 13-plex immune-oncology panel and images it in a single round on RareCyte’s Orion spatial biology platform, then phenotypes the cells at single-cell resolution and maps their spatial neighborhoods in Visiopharm’s Phenoplex.
Presented by Visiopharm A/S and RareCyte.
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- A whole-slide colon adenocarcinoma stained once and imaged in a single round. A standard pathology slide was stained with a 13-plex immune-oncology panel, then a 21.6 by 18.5 mm section was imaged in one round on RareCyte’s Orion spatial biology platform — capturing the whole tissue at single-cell resolution without the repeated staining steps that multi-round high-plex imaging otherwise requires.
- Single cells read straight from the tissue and mapped to their compartments. Visiopharm’s Phenoplex segmented the whole-slide image into epithelial, stromal, and necrotic regions, called individual cells from their DAPI-stained nuclei, and phenotyped tumor, T-cell, B-cell, and macrophage populations across the 13-plex panel — finding many T cells in the stroma but few infiltrating the epithelium.
- Spatial neighborhoods that separate PD-L1-positive from PD-L1-negative macrophages. Within the stromal region, nearest-neighbor and neighbor-count analyses out to 30 µm showed that PD-L1+ macrophages occupy a different immune-cell neighborhood than PD-L1- macrophages — clustering with T cells and other macrophages while sitting farther from proliferative and regulatory T cells.
